2021
Judul/Title |
Dynamic change in bacterial communities in the integrated rice–fish farming system in Sleman, Yogyakarta, Indonesia
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Penulis/Author | Afri Herlambang, Murwantoko Murwantoko, Indah Istiqomah |
Tanggal/Date | 2021 |
Abstrak/Abstract | An integrated rice–fish farming system is an efficient technique used to maintain water quality and rice/fish productivity. In addition to fish and rice plants that have mutually beneficial ecological roles, bacteria play important roles in maintaining water quality and soil fertility. Therefore, this study investigated the diversity and dynamic change in the bacterial community composition of water and sediment in an integrated rice–fish farming system using a high-throughput sequencing approach. The 16S rRNA gene of bacteria was amplified, and the IonS5™XL sequencing platform was used to identify bacterial community during the cultivation period. The results demonstrated that fishpond, rice field and trench sediment samples were dominated by the phyla Proteobacteria (60.9%–64.4%), Acidobacteria (8.7%) and Chloroflexi (6.3%), whereas water samples were dominated by the phyla Proteobacteria (65.7%), Bacteroidetes (17.1%) and Actinobacteria (8.5%). The dynamics of the bacterial community composition of the water and sediment samples were clearly observed at phylum and genus levels respectively. Water temperature (34.67°C) and ammonia-nitrogen content (0.38 mg L−1) at the early stage of cultivation correlated with the abundances of Actinobacteia and Cyanobacteria at the phylum level and Acinetobacter at the genus level. Furthermore, several bacterial genera could potentially contribute to the biogeochemical cycle. In particular, Sorangium was involved in organic matter degradation, Methylophilus was implicated in nitrification, Novosphingobium and Methylotenera were essential for denitrification, and Desulfobacca was important for sulfate reduction. This study improved our understanding of the bacterial community in the integrated rice–fish farming system and provided data for further development. |
https://doi.org/10.1111/are.15432
PKM activities in the form of management and cultivation technical training are carried out routinely on Wednesday nights at the end of each month and incidentally as needed (Figure 1). Some of the training topics include: strengthening Pokdakan management, good fish hatchery methods (CPIB), good fish farming practices (CBIB), aquaculture business planning, catfish and gouramy hatchery systems, catfish and gouramy rearing systems, etc. This activity was also supported by several student researches taking topics and places in the MDS Pokdakan. As a follow-up to these activities, Pokdakan MDS has registered the Pre-CBIB Competition at the DIY Province level. The evaluation of the competition by the Department of Maritime Affairs and Fisheries of the DIY Province took place on September 6, 2020 (Figure 2). Currently, Pokdakan MDS is improving documents, compiling Standard Operating Procedures (SOP), and improving cultivation performance in order to prepare for CBIB certification.
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Judul/Title | Chemical composition diversity of fucoidans isolated from three tropical brown seaweeds (Phaeophyceae) species |
Penulis/Author | FADILAH NORLAILI LUTFIA (1); Dr. Ir. Alim Isnansetyo, M.Sc. (2) ; Prof. Dr. Ratna Asmah Susidarti, MS., Apt. (3); Muhammad Nursid (4) |
Tanggal/Date | 4 2020 |
Abstrak/Abstract | Abstract. Lutfia FN, Isnansetyo A, Susudarti RA, Nursid M. 2020. Chemical composition diversity of fucoidans isolated from three tropical brown seaweeds (Phaeophyceae) species. Biodiversitas 21: 3170-3177. Fucoidan is a polysaccharide with high sulfate content, found in the cell walls matrix of brown seaweed. Its bioactivities vary depending on the algae species and the chemical structure. The aims of this research were to extract, purify, and characterize fucoidan from three Indonesian brown seaweed, Sargassum sp., Turbinaria sp., and Padina sp. The extraction was carried out using acid method followed by precipitation with ethanol and CaCl2, while its purification using DEAE-cellulose ion-exchange chromatography. Characterization was performed by FTIR and 1H-NMR spectroscopic analyses. Chemical components of fucoidan determined were total carbohydrates, sulfate residue, uronic acid residue, and monosaccharide components. The results revealed that the Turbinaria sp. produced highest yield of fucoidan (4.8% dry matter), followed by Sargassum sp. (2.7% dry matter) and Padina sp. (2.6% dry matter). The carbohydrate contents of Sargassum sp., Turbinaria sp. and Padina sp. fucoidan were 64.55±0.12%, 67.42 ± 0.03% and 62.90 ± 0.04% with sulfate residues of 0.013% ± 4.71; 19.47±0.002% and 0.016%±8.81; and uronic acid residues of 25.19±0.03%; 12.69±0.03% and 12.91±0.01%, respectively. Sargassum sp., Turbinaria sp., and Padina sp. fucoidans consist of fucose and some other minor monosaccharides. |
Judul/Title | Effects of Supplementation with Furikake Ulvamina Made of Algae (Ulva sp.) and Tuna (Thunnini) on Cognitive Function of Malnourished Mice (Rattus norvegicus) |
Penulis/Author | Dr. Toto Sudargo, SKM., M.Kes. (1) ; Dr. Ir. Alim Isnansetyo, M.Sc. (2); Indun Dewi Puspita, S.P., M.Sc. Ph.D. (3); Dr. R.A. Siti Ari Budhiyanti, S.T.P., M.P. (4); Rahadyana Muslichah, S.Gz., M.Sc. (5); TIRA ARISTASARI (6); SHEILA ROSMALA PUTRI (7); KHUSNUL ALFIONITA (8); Yosephin Anandati Pranoto, S.Gz., M.S., R.D. (9) |
Tanggal/Date | 10 2020 |
Abstrak/Abstract | Algae (Ulva sp.) and tuna (Thunnini) are edible marine resources rich in protein, essential amino acids, zinc, and iodine that can be used to improve the growth of malnourished children including their cognitive functions. The combination of both resources may produce a supplementation (Furikake Ulvamina) with nutritional quality to support the acceleration of cognitive functions of malnourished children. This study aimed to develop a marine-based product/supplement to accelerate cognitive functions in malnourished mice (Rattus norvegicus). A product called “Furikake Ulvamina” was used in an experimental study of 25 malnourished mice. The Morris Water Maze test was adapted into a “speed time to survive” to test the cognitive functions of each group. One-way Anova tests were performed to statistically analyze the mean differences in the speed of time to survive before and after the intervention. All five groups showed an increase in the speed of time to survive after treatment was given with an average increase of speed of 26.9 seconds. Statistically, there was no significant difference in this cognitive function acceleration indicator between groups (p>0.05). Furthermore, weight and length were also increased after four weeks in all groups. There was no significant difference in cognitive function acceleration in each group of mice after the supplementation of Furikake Ulvamina for four weeks. This product may potentially accelerate growth in malnourished mice. Furikake Ulvamina can also promote the consumption and benefits of Indonesian marine local commodities (algae and tuna). |
Judul/Title | Morphometric variation of Cork fish (Channa striata Bloch, 1793) from nine populations in Sumatra Island, Indonesia |
Penulis/Author | BOBY MUSLIMIN (1); Prof. Dr. Ir. Rustadi, M.Sc. (2); Dr. Ir. Ignatius Hardaningsih, M.Si. (3); Dr. Bambang Retnoaji, M.Sc. (4) |
Tanggal/Date | 7 2020 |
Abstrak/Abstract | The geographic isolation and specific character of local habitat could cause variation of morphological characteristics among Cork fish population in different locations. The plasticity of cork fish to adapt to the environment factors possibly has an impact on the different of specific morphological on fish population at different habitat. This study was conducted to investigate the traditional morphometric measurement and truss network differentiation of cork fish populations in different locations at Sumatra Island. Total of 394 cork fish specimens (consisting of 198 males and 196 females) were collected from nine different areas of rivers, swamp and lakes. The samples were analysed based on 14 morphometric (M) and 21 Truss Network Measurement (TNM) characters to find out the significant correlation of the fish on each area sampling. The transformed datasets were proceeded to multivariate testing using Discriminant Function Analysis (DFA) and Cluster Analysis (CA). The results showed that cork fish had 20 distinguishing predominant characters in the head and back of the body. Based on cluster analysis, those fishes were divided into 4 groups depending on geographical isolation except one floodplain population that differs from the other population groups. We revealed that phenotypic dimorphism for female cork fish had a dominant size compared to males in head region and tail fin region. Cork fish in the lake had a dominant body height size compared to rivers and flooded swamps. |
Judul/Title | Viability of Najawa carp (Cyprinus carpio L.) sperm at 4°C temperature storage |
Penulis/Author | Dimas Fendy Pradana (1); Dr. Ir. Ignatius Hardaningsih, M.Si. (2); Dr. Dini Wahyu Kartika Sari, S.Pi., M.Si. (3) |
Tanggal/Date | 10 2020 |
Abstrak/Abstract | The objectives of this study were to evaluate the sperm viability of Najawa carp (Cyprinus carpio L.) in cryopreservation pre-conditions at 4°C. The design used in this study was Complete Randomized Design with 4 treatments, BSS as a control, 10% DMSO, 0,2 M Sucrose, and 5% DMSO + 0,1 M Sucrose; each consist of three replications. The parameters observed were progressive motility of fresh sperm, diluted sperm before low temperature storage, and 2 hours; 3 hours; 4 hours; 5 hours; one day; one week; a month after 4°C storage. The data were analyzed by ANOVA. The data showed that there was no significant difference between treatment (P>0.05). The best viability was 40.56% of sperm motility which survive for one week, it was achieved by 5% DMSO + 0,1 M Sucrose. |