| Time Frame for Technology Research and Development | ||||
|
Already Done (2016-2019) |
Year I (2020) | Year II (2021) | Year III (2022) | |
| Problem | Bacterial disease attack on fish and shrimp, survival rate and growth is not optimal | It is necessary to study the effective way of application (frequency) | · It is necessary to study the storage and packaging of probiotic products
· It is necessary to study the efficacy of probiotic application in shrimp |
It is necessary to study the effect of packaging and storage (according to the results of year II) on the efficacy of probiotics on red tilapia on a laboratory and field scale, as well as product socialization to the public. |
| Research for Problem Solving | Studying methods of developing biocontrol systems, isolation and characterization of bacteria from fish intestines as probiotic candidates | Application of probiotics with a combination of dose and frequency, with observations of growth, immunity, immune gene expression in the gut, fish resistance to infection, FCR and gut microbial metagenomes | · Test storage and packaging of probiotics with various microencapsulants
· Periodic evaluation of bacterial survival in various microencapsulants · Test the application of probiotics on white shrimp |
· Test the application of packaged probiotic products (according to the results of year II) on red tilapia and shrimp on a laboratory and field scale
· Socialization of the application of packaged probiotic products (according to the results of year II) to red tilapia and shrimp farmers |
| Scientific Output | Understanding of probiotic development methods | Understanding of the best frequency of application to get the best impact on value | · Understanding of the best storage and packaging of probiotics
· Understanding of the efficacy of probiotic application on white shrimp |
Proof that the packaging and storage of probiotics has a positive effect on the performance of red tilapia cultivation on a laboratory and field scale, as well as the recognition of probiotic products in this study by red tilapia and shrimp farming business actors. |
| Technological/
Product Output |
4 potential probiotic candidates were obtained | Obtained the best patented probiotic formula and frequency of application | · Obtained the best probiotic storage method that can be patented and published
· Obtained data on the efficacy of probiotic applications in shrimp |
Discovery of probiotic technology packages for red tilapia cultivation, including formulas, packaging and storage methods, application methods to fish and shrimp. |
