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Time Frame for Technology Research and Development |
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Already Done (2016-2019) |
Year I (2020) |
Year II (2021) |
Year III (2022) |
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| Problem | Bacterial disease attack on fish and shrimp, survival rate and growth is not optimal | It is necessary to study the effective way of application (frequency) | It is necessary to study the storage and packaging method of probiotic products, to study the efficacy of packaged probiotics, to study the in vivo activity of nitrifying bacteria, and to study pathogenic bacteria in vaname shrimp and abalone. | It is necessary to study the effect of packaging and storage (according to the results of year II) on the efficacy of probiotics on red tilapia on a laboratory and field scale, as well as product socialization to the public. |
| Research for Problem Solving | Studying methods of developing biocontrol systems, isolation and characterization of bacteria from fish intestines as probiotic candidates | Application of probiotics with a combination of dose and frequency, with observations of growth, immunity, immune gene expression in the gut, fish resistance to infection, FCR and gut microbial metagenomes | · Test storage and packaging of probiotics with various microencapsulants, and periodic viability evaluation
· prepackaged probiotic efficacy test, · in vivo efficacy test of nitrifying bacteria, · characterization of pathogenic bacteria in vaname shrimp and abalone |
· Test the application of packaged probiotic products (according to the results of year II) on red tilapia on a laboratory and field scale
· Socialization of the application of packaged probiotic products (according to the results of year II) to red tilapia cultivators |
| Scientific Output | Understanding of probiotic development methods | Understanding of the best frequency of application to get the best impact on value | Understanding of the best storage and packaging of probiotics, efficacy of packaged probiotics, nitrifying bacteria eviction, pathogenic characters in vaname shrimp and abalone | Proof that the packaging and storage of probiotics has a positive effect on the performance of red tilapia cultivation on a laboratory and field scale, as well as the recognition of probiotic products in this study by red tilapia cultivation business actors. |
| Technological/Product Output | 4 potential probiotic candidates were obtained | Obtained the best patented probiotic formula and frequency of application | Obtained the best patented probiotic storage method | The discovery of probiotic technology packages for red tilapia cultivation, including formulas, packaging and storage methods, application methods to fish. |
