Judul/Title | Chemical composition diversity of fucoidans isolated from three tropical brown seaweeds (Phaeophyceae) species |
Penulis/Author | FADILAH NORLAILI LUTFIA (1); Dr. Ir. Alim Isnansetyo, M.Sc. (2) ; Prof. Dr. Ratna Asmah Susidarti, MS., Apt. (3); Muhammad Nursid (4) |
Tanggal/Date | 4 2020 |
Abstrak/Abstract | Abstract. Lutfia FN, Isnansetyo A, Susudarti RA, Nursid M. 2020. Chemical composition diversity of fucoidans isolated from three tropical brown seaweeds (Phaeophyceae) species. Biodiversitas 21: 3170-3177. Fucoidan is a polysaccharide with high sulfate content, found in the cell walls matrix of brown seaweed. Its bioactivities vary depending on the algae species and the chemical structure. The aims of this research were to extract, purify, and characterize fucoidan from three Indonesian brown seaweed, Sargassum sp., Turbinaria sp., and Padina sp. The extraction was carried out using acid method followed by precipitation with ethanol and CaCl2, while its purification using DEAE-cellulose ion-exchange chromatography. Characterization was performed by FTIR and 1H-NMR spectroscopic analyses. Chemical components of fucoidan determined were total carbohydrates, sulfate residue, uronic acid residue, and monosaccharide components. The results revealed that the Turbinaria sp. produced highest yield of fucoidan (4.8% dry matter), followed by Sargassum sp. (2.7% dry matter) and Padina sp. (2.6% dry matter). The carbohydrate contents of Sargassum sp., Turbinaria sp. and Padina sp. fucoidan were 64.55±0.12%, 67.42 ± 0.03% and 62.90 ± 0.04% with sulfate residues of 0.013% ± 4.71; 19.47±0.002% and 0.016%±8.81; and uronic acid residues of 25.19±0.03%; 12.69±0.03% and 12.91±0.01%, respectively. Sargassum sp., Turbinaria sp., and Padina sp. fucoidans consist of fucose and some other minor monosaccharides. |
Arsip 2021:
January
Judul/Title | Effects of Supplementation with Furikake Ulvamina Made of Algae (Ulva sp.) and Tuna (Thunnini) on Cognitive Function of Malnourished Mice (Rattus norvegicus) |
Penulis/Author | Dr. Toto Sudargo, SKM., M.Kes. (1) ; Dr. Ir. Alim Isnansetyo, M.Sc. (2); Indun Dewi Puspita, S.P., M.Sc. Ph.D. (3); Dr. R.A. Siti Ari Budhiyanti, S.T.P., M.P. (4); Rahadyana Muslichah, S.Gz., M.Sc. (5); TIRA ARISTASARI (6); SHEILA ROSMALA PUTRI (7); KHUSNUL ALFIONITA (8); Yosephin Anandati Pranoto, S.Gz., M.S., R.D. (9) |
Tanggal/Date | 10 2020 |
Abstrak/Abstract | Algae (Ulva sp.) and tuna (Thunnini) are edible marine resources rich in protein, essential amino acids, zinc, and iodine that can be used to improve the growth of malnourished children including their cognitive functions. The combination of both resources may produce a supplementation (Furikake Ulvamina) with nutritional quality to support the acceleration of cognitive functions of malnourished children. This study aimed to develop a marine-based product/supplement to accelerate cognitive functions in malnourished mice (Rattus norvegicus). A product called “Furikake Ulvamina” was used in an experimental study of 25 malnourished mice. The Morris Water Maze test was adapted into a “speed time to survive” to test the cognitive functions of each group. One-way Anova tests were performed to statistically analyze the mean differences in the speed of time to survive before and after the intervention. All five groups showed an increase in the speed of time to survive after treatment was given with an average increase of speed of 26.9 seconds. Statistically, there was no significant difference in this cognitive function acceleration indicator between groups (p>0.05). Furthermore, weight and length were also increased after four weeks in all groups. There was no significant difference in cognitive function acceleration in each group of mice after the supplementation of Furikake Ulvamina for four weeks. This product may potentially accelerate growth in malnourished mice. Furikake Ulvamina can also promote the consumption and benefits of Indonesian marine local commodities (algae and tuna). |
Judul/Title | Morphometric variation of Cork fish (Channa striata Bloch, 1793) from nine populations in Sumatra Island, Indonesia |
Penulis/Author | BOBY MUSLIMIN (1); Prof. Dr. Ir. Rustadi, M.Sc. (2); Dr. Ir. Ignatius Hardaningsih, M.Si. (3); Dr. Bambang Retnoaji, M.Sc. (4) |
Tanggal/Date | 7 2020 |
Abstrak/Abstract | The geographic isolation and specific character of local habitat could cause variation of morphological characteristics among Cork fish population in different locations. The plasticity of cork fish to adapt to the environment factors possibly has an impact on the different of specific morphological on fish population at different habitat. This study was conducted to investigate the traditional morphometric measurement and truss network differentiation of cork fish populations in different locations at Sumatra Island. Total of 394 cork fish specimens (consisting of 198 males and 196 females) were collected from nine different areas of rivers, swamp and lakes. The samples were analysed based on 14 morphometric (M) and 21 Truss Network Measurement (TNM) characters to find out the significant correlation of the fish on each area sampling. The transformed datasets were proceeded to multivariate testing using Discriminant Function Analysis (DFA) and Cluster Analysis (CA). The results showed that cork fish had 20 distinguishing predominant characters in the head and back of the body. Based on cluster analysis, those fishes were divided into 4 groups depending on geographical isolation except one floodplain population that differs from the other population groups. We revealed that phenotypic dimorphism for female cork fish had a dominant size compared to males in head region and tail fin region. Cork fish in the lake had a dominant body height size compared to rivers and flooded swamps. |
Judul/Title | Viability of Najawa carp (Cyprinus carpio L.) sperm at 4°C temperature storage |
Penulis/Author | Dimas Fendy Pradana (1); Dr. Ir. Ignatius Hardaningsih, M.Si. (2); Dr. Dini Wahyu Kartika Sari, S.Pi., M.Si. (3) |
Tanggal/Date | 10 2020 |
Abstrak/Abstract | The objectives of this study were to evaluate the sperm viability of Najawa carp (Cyprinus carpio L.) in cryopreservation pre-conditions at 4°C. The design used in this study was Complete Randomized Design with 4 treatments, BSS as a control, 10% DMSO, 0,2 M Sucrose, and 5% DMSO + 0,1 M Sucrose; each consist of three replications. The parameters observed were progressive motility of fresh sperm, diluted sperm before low temperature storage, and 2 hours; 3 hours; 4 hours; 5 hours; one day; one week; a month after 4°C storage. The data were analyzed by ANOVA. The data showed that there was no significant difference between treatment (P>0.05). The best viability was 40.56% of sperm motility which survive for one week, it was achieved by 5% DMSO + 0,1 M Sucrose. |
Judul/Title | Replacement of fish meal with fermented Moringa leaves meal and its effect on the growth performance of red tilapia (Oreochromis sp.) |
Penulis/Author | Dr. Senny Helmiati, S.Pi., M.Sc. (1); Prof. Dr. Ir. Rustadi, M.Sc. (2); Dr. Ir. Alim Isnansetyo, M.Sc. (3) ; Prof. Dr. Ir. Zuprizal, DEA. (4) |
Tanggal/Date | 26 2020 |
Abstrak/Abstract | The purpose of the present research was to evaluate the nutritional content of fermented Moringa oleifera leaves meal and to find out the effect of fish meal replacement with fermented Moringa leaves meal (FMLM) in diets on the growth performance of red tilapia (Oreochromis sp.). The treatments include replacement of the fish meal with FMLM at 0, 10, 20, and 30%. Red tilapia with total length of 9-10 cm was fed twice daily for two months at a feeding rate of 3% of total biomass. The results revealed that fermentation increased the moisture, protein, ash, total lipid, calcium, and phosphorous content in Moringa leaves meal (MLM). The fermentation process actually reduced the anti-nutrient substances (phenol, tannin, phytic acid, and hydrogen cyanide (HCN)) in MLM. Fish meal replacement with FMLM in diets significantly affected feed digestibility. FMLM can be used as a replacement for fish meal in diet at 30% with only limited effect on the growth performance of red tilapia |
Judul/Title | Protective Effect of Microbubble Aeration and Dietary Probiotics BALSS on Survival and Immunity of White Leg Shrimp (Litopenaeus vannamei) Postlarvae against Acute Low Salinity Stress |
Penulis/Author | Indah Istiqomah, S.Pi., M.Si., Ph.D (2) ; Prof. Dr. Ir. Rustadi, M.Sc. (3); Dr. Ir. Bambang Triyatmo, M.P. (4); Dr. Ir. Alim Isnansetyo, M.Sc. (5); Wiratni, S.T., M.T., Ph.D. (6); Dr. Deendarlianto, S.T., M.Eng. (7) |
Tanggal/Date | 30 2020 |
Abstrak/Abstract | Survival rate and immunity of white leg shrimp (Litopenaeus vannamei) postlarvae against acute salinity stress were evaluated in indoor experimental trials. The research was conducted with 2×2 factorial design with two levels of aeration (microbubble and macro bubble) and two levels of diet (with and without probiotics) resulting four treatments in triplicate: application of microbubble with gut probiotics (MiP), without gut probiotics (MiC), and macro bubble with gut probiotics (MaP) and without probiotics (MaC). White leg shrimps measuring 7.6 g were maintained in the tanks for 60 days and fed five times a day at a dose of 5% biomass. Harvested shrimp were tested for low salinity stress (5 ppt) for 3 hours. Glucose level, total protein, bactericidal activity, natural agglutination, phenoloxidase, respiratory burst, and superoxide dismutase activities in the hemolymph and the shrimp survival rate (SR) were analyzed before and after the stress tests. Although all examined parameters remained the same between groups before the stress test, significant differences were found among them after the stress test. Microbubble aeration obtained stabilization of hemolymph glucose level, best survival rate, bactericidal activity, and agglutination activities. No significant effect was resulted by the dietary probiotic treatments, but the interaction with aeration treatments revealed the importance to compensate shrimp survival rate when microbubble aeration is unavailable. |
Judul/Title | An Evaluation of Nutrient Value and Antinutritional Contents of Fermented Moringa oleifera Leaves Meal as Fish Feed Ingredient |
Penulis/Author | Dr. Senny Helmiati, S.Pi., M.Sc. (1) ; Prof. Dr. Ir. Rustadi, M.Sc. (2); Dr. Ir. Alim Isnansetyo, M.Sc. (3); Prof. Dr. Ir. Zuprizal, DEA. (4) |
Tanggal/Date | 30 2020 |
Abstrak/Abstract | The aim of the study was to evaluate nutritive value and antinutritional contents of fermented Moringaoleifera leaves meal as a plant-based protein source as an alternative ingredient for aquafeed. The research stages included processing of M. oleifera leaf meal, preparation and calculation of bacterial density, fermentation process, and analysis of nutrient and antinutrient content. The bacteria used for fermentation is a mixture of T2A (Bacillus sp.), T3P1 (Bacillus sp.) and JAL11 (Lactococcus raffinolactis) bacteria with a density of 2.16×109 cfu/mL. Fermented M. oleifera leaves meal was carried out for 168 hours. Analysis of nutrient content and antinutrient substances of fermented M. oleifera leaves meal was carried out at 24, 48, 72, 96, 120, 144 and 168 hours. The nutrient content of Moringa leaf meal includes moisture (9.04±0.00%), ash (9.70±0.21%), protein (25.77±0.08%), fat (4.80± 0.52%), crude fiber (11.60±0.13%), extract material without nitrogen (39.06±0.52%), energy (351.27±3.27 kcal/100 g), hemicellulose (13.79±0.07%), cellulose (9.9±0.06%) and lignin (15.34±0.31%). Fermentation can increase moisture, ash, protein and fat content, and reduce crude fiber content, nitrogen free extract, hemicellulose, cellulose, lignin and antinutrients, including phenols, tannins, phytic acid and HCN. The results of the present study show that fermentation is able to improve nutrient dan decrease antinutritional content of M. oleifera leaves meal as a plant-based protein source for further use in aquafeed ingredient. |
Judul/Title | Buku Putih Universitas Gadjah Mada: Gagasan Bulaksumur Membangun Kedaulatan Pangan Nusantara Inspirasi UGM untuk Indonesia |
Penulis/Author | Dr. Jamhari, S.P., M.P. (1); Prof. Dr. Ir. Umar Santoso, M.Sc. (2); R. Ahmad Romadhoni Surya Putra, S.Pt., M.Sc., Ph.D. (3); Prof. Dr. Ir. Rustadi, M.Sc. (4); Subejo, S.P., M.Sc., Ph.D. (5); Agus Dwi Nugroho, S.P., M.Sc. (6); Mesalia Kriska, S.P., M.Sc. (7); Arif Wahyu Widada, S.P., M.Sc. (8); Muslimin, S.P., M.P. (9) |
Tanggal/Date | 2019 |
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Judul/Title | MERANGKAI GAGASAN DAN INOVASI: Merespon Pandemi Covid-19 untuk Kemajuan Perikanan dan Kelautan |
Editor | Dr. Ir. Alim Isnansetyo, M.Sc.
Dr. Amir Husni, S.Pi., M.P. Dr. Eko Setyobudi, S.Pi., M.Si. Dr. Ratih Ida Adharini, S.Pi., M.Si. Mgs. Muhammad Prima Putra, S.Pi., M.Sc., Ph.D. |
Tanggal/Date | 2020 |
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Judul/Title | Manajemen Budidaya Udang dalam Tambak yang Ramah Lingkungan dan Berkelanjutan saat dan Pasca Pandemi Virus Corona 2019 (covid-19) |
Penulis/Author | Dr. Ir. Bambang Triyatmo, M.P. (1) |
Tanggal/Date | 10 2020 |
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